Long-Read Sequencing by SMARTLife — Complete Analysis of Plasmids and Amplicons

Our Long-Read Sequencing service offers a powerful and reliable solution for the comprehensive analysis of plasmids and PCR amplicons, delivering accurate, full-length sequences with exceptional clarity. Unlike short-read technologies, our approach eliminates gaps and ambiguities, enabling in-depth insight into your DNA constructs—whether you're working with circular plasmids, linear fragments, or targeted genomic regions.

Thanks to advanced long-read technology, SMARTLife ensures superior resolution of complex genetic structures, repetitive sequences, and functional elements that are often missed or misassembled with traditional sequencing methods.

Two Applications, One Powerful Technology

✅ Plasmid Sequencing

Ideal for synthetic biology, microbiology, and genetic engineering, this service delivers complete plasmid sequences in a single read. Our analysis covers:

• Full-length circular or linear plasmids

• Structural variants, gene rearrangements, and complex regions

• Precise annotation of features such as antibiotic resistance genes, replication origins, and regulatory elements

• Superior assembly quality with minimal gaps

✅ Amplicon/PCR Product Sequencing

Perfect for studying specific genes, variants, or genomic regions, our long-read sequencing captures your entire PCR product, no matter the complexity:

• Uninterrupted sequencing of full-length amplicons

• High-resolution mutation detection (SNPs, indels, structural variants)

• Improved analysis of repetitive or homopolymeric regions

• Tailored for applications in gene variant detection, microbiome studies, and targeted sequencing

Why Choose SMARTLife Long-Read Sequencing?

• Complete and accurate results: High-quality, full-length reads without assembly errors

• Faster turnaround: Results delivered within 24 hours

• Scalable: Process multiple samples in parallel for high-throughput projects

• Comprehensive bioinformatics: Clear, actionable results backed by expert analysis

Deliverables

Each sequencing project includes:

• Raw data (.fastq files with quality scores)

• Consensus sequence (.fasta) generated via our proprietary auto-assembly pipeline

• Base-by-base quality and coverage tables (.tsv and .xlsx)

• For plasmids: graphical map and annotations (.pLannote file)

• For both applications: alignment files (.bam) and variant tables comparing your sequence to a provided reference (.tsv and .xlsx)

Work With a Local Team That Listens

SMARTLife combines cutting-edge sequencing technology with human expertise. Our local team is ready to support your research with responsive service, personalized advice, and clear reporting. Whether you're characterizing plasmids for therapeutic development or sequencing complex amplicons for microbial or genetic studies, we deliver the depth, precision, and speed your project demands.