CRISPR SMARTSEQ
Get the complet sequence of your construct
Choose the best sequencing service for your CRISPR construct and order now.
We can sequence entire sgRNA vector to help you analyze your CRISPR/Cas library. By adjusting the sequencing coverage (REGULAR 30X or DEEP 100X), our sequencing CRISPR service is appropriate for all of your needs. By sequencing your construct, you can manage fine mapping loci and resolve single-nucleotide and haplotype variants at the haplotype level.
We offer you different CRISPR SMARTsequencing services.
CRISPR sgRNA Vector SMARTSeq
CRISPR Amplicon SMARTSeq REGULAR (30X)
CRISPR Amplicon SMARTSeq DEEP
(100X)
- Valid for CRISPR/ Cas circular construct up to 25 kb
- Dedicated protocol valid for circular double stranded DNA
- Available from 1 sample to 96 samples
- High quality of results (99.6% accuracy)
- 30X sequencing coverage minimum
- Verify your sgRNAs construct library
- Get the complete sequence of your circular construct
- Detect all variants : from SNPs to large structural variation
- Available from 1 sample to 96 samples
- Sequencing of up to 25 kb CRISPR/Cas linear amplicon
- REGULAR (30X) sequencing coverage for routine mapping
- Adjusted protocol with high quality base-calling dedicated to variants detection
- Full length sequence of targeted loci
- Save cloning time & sequence directly your CRISPR Amplicons
- Effective transgene mapping with complex integration loci
- Detect all classes of variants , from SNPs to complex non-coding structural variants
- Available from 1 sample to 96 samples
- Sequencing of up to 25 kb CRISPR/Cas linear amplicon
- DEEP (100X) sequencing coverage for precise visualization of targeted loci and clivage sites
- Adjusted protocol with high quality base-calling dedicated to variants detection
- Full length sequence of targeted loci
- Save cloning time & sequence directly your CRISPR amplicons (up to 25 kb)
- Effective transgene mapping with complex integration loci
- Detect all classes of variants : from SNPs to complex non-coding structural variants
Secure the success of your CRISPR/Cas gene editing experiment.
Verify upfront the complete sequence of your sgRNA construct by long read sequencing.
How to order
It is very easy to order your samples sequencing online.
CRISPR SMARTSeq step by step :
- Prepare your purified double strand circular vector or amplicon in line with our concentration & volume requirements
- Label each sample with Green Long read SMARTSeq IDs
- Connect to your SMARTAccount online
- Select QUICK ORDER / Long read
- Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
- Protect your samples in a SMARTBox and write on the back of the box your name and order number
- Contact us for local pick up support@smartlifebiosciences.com
Results
For CRISPR/Cas project, SMARTLife Biosciences produces REGULAR (30X) or DEEP (100X) sequencing coverage and long reads (up to 300 kb) . Long reads are aligned to the reference genome with a proprietary bio-IT pipeline. You receive in your personal & secure One Drive the following results:
- Raw data (text sequences & quality scores (fastq. file)
- Consensus sequence (.fasta file)
- Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
- Table of detected variants compared to the reference sequence (.vcf file)
- Quality metrics & histograms
Discover as well