LINEAR/AMPLICON SMARTSEQ

LINEAR/AMPLICON SMARTSEQ


  • 3d generation sequencing technology
  • No need of sequencing primers
  • Amplification free, real time data acquisition
  • No sample transportation, local laboratories
  • Detect all variants : from SNPs to structural variation
  • Proprietary BioIT pipelines
  • Custom BioIT services available on request

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Linear / Amplicon SMARTSEQ step by step :

  1. Prepare your purified linear DNA / amplicon PCR in line with our concentration & volume requirements
  2. Label your tube with a Blue long read SMARTSeq lDs.
  3. Connect to your SMARTAccount online.
  4. Select QUICK ORDER / Long read.
  5. Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
  6. Protect your samples in a SMARTBox and write on the back of the box your name and order number.
  7. Contact  us for local pick up support@smartlifebiosciences.com

SMARTLife Biosciences produces long reads (30X coverage) and performs an alignment to your reference sequence with a proprietary bio-IT pipeline. You receive in your personal & secure One Drive the following results:

  1. Raw data (text sequences & quality scores (fastq. file)
  2. Consensus sequence (.fasta file)
  3. Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
  4. Table of detected variants compared to the reference sequence (.vcf file)
  5. Quality metrics & histograms
Visualisation of bases quality score with SnapGene Viewer
Variants detected by SMRT sequencing on the left panel (multiple sequences and high sequencing depth at the mutation site)
compared to the conventional Sanger method on the right panel

Don’t limit yourself .
Obtain the COMPLETE sequence of amplicon PCR.
Detect all variants with 3d generation long read sequencing.