Oligonucleotides

Custom DNA/ RNA Oligos
Modifications
Specific Oligos

 

Trust SMARTLife Biosciences for high-quality oligos that will support the success of your research and innovations!

At SMARTLife Biosciences, we take pride in delivering the highest quality oligonucleotides for all your biological applications. With our cutting-edge expertise and advanced purification technologies such as HPLC and OPC®, we guarantee products perfectly tailored to your most demanding needs.

Whether for PCR, cloning, sequencing, or FISH applications, our oligos are synthesized with unmatched precision, ensuring reliable and reproducible results. We can fulfill all your requests, from short oligos to the longest (up to 220 bp), with or without specific modifications (5′, 3′, or internal).

Our oligonucleotides are rigorously controlled at every stage of production, offering you products of exceptional purity, ready for use in your most sensitive projects.

Choose SMARTLife Biosciences

                    • Ultra high quality oligos
                    • Oligos dry delivered in single tube
                    • Delivered directly in your dropbox by our local SMART Team
                    • 4 synthesis scales available : 0.02; 0.04; 0.2; 1 µmol.
                    • Large panel of modifications (all DNA oligos have terminal hydroxyl groups at both ends)
                    • Small or large-scales DNA oligos available
                    • QCs done on mass spectrometry
                    • Guaranteed yield expressed in OD (calculated for unmodified oligonucleotides (20mers))
                    • Purification available: desalted/ HPLC/ OPC

                                  Custom DNA / RNA oligos

                                  DESALTED

                                  Desalting helps to remove most of the low molecular weight by-products that form and accumulate during chemical synthesis reactions. This purification method is suitable for oligonucleotides of less than 30 nt and/or those intended for routine, low-criticality laboratory applications, such as PCR, sequencing, probing, mobility shift, or hybridization. We would like to emphasize that desalted oligonucleotides are not recommended for molecular cloning projects, for which oligonucleotides purified by superior methods, such as HPLC, are advised.

                                  HPLC PURIFICATION

                                  HPLC purification (high-performance liquid chromatography) is recommended for oligos longer than 80 nt and for all more sensitive laboratory procedures. This purification method allows for the removal of truncated oligos and residual products. HPLC is also particularly useful for achieving higher purity, which is necessary for successful applications that require higher sensitivity, such as cloning, DNA fingerprinting, real-time PCR, FISH, etc.

                                  OPC PURIFICATION

                                  This purification method consists of reverse-phase chromatography (RP), which separates the complete, newly synthesized oligonucleotides from defective sequences, by-products, and other impurities. More specifically, the OPC® cartridge binds the dimethoxytrityl (DMT) group of the oligonuclotide carrying the trityl (complete, newly synthesized) from molecules that do not carry the DMT. This technique is particularly effective for oligonucleotides up to 40 nucleotides in length.

                                  Modifications

                                  Fluorescente Modifications

                                  Non Fluorescente Modifications

                                  Specific oligos, primers, and probes

                                  OLIGOS

                                  • Phosphorothiorate (PTO)
                                  • 2’OMeRNA
                                  • Chimères

                                  PRIMERS

                                  • ZNA
                                  • LNA

                                  PROBES

                                  • Dual Labelled Fluorogenic Probes (DLPs)
                                  • ZNA
                                  • MGB
                                  • Double Quenched
                                  • Light Cycler (LC) HybProbes
                                  • LNA

                                  Please contact us at support@smartllifebiosciences.com for any pricing inquiries regarding specific oligos, primers, and probes.