LOW PASS
GENOME SEQUENCING
Genome characterization by Low pass Long-read sequencing
Low pass Genome long read sequencing is a very attractive and cost-effective approach for genome characterization. It requires a low sequencing depth (1X to 5X) to be cost effective. Low-pass whole genome sequencing (also referred to as shallow whole genome sequencing) is used to detect or characterize genetic variation. This method is an affordable alternative for discovering new variants with higher specificity due to long read data. Low Pass Genome Long read sequencing helps to address questions related to statistical and population genetics, from complex-trait association studies to genome-wide polygenic risk score calculation.
Shallow WGS returns more specific data, greater and new variant discovery capabilities than traditional genotyping arrays or shallow WGS short reads sequencing. Shallow WGS can be used in genome-wide association study (GWAS), evolutionary analysis, pharmacogenomics, molecular breeding, etc. In addition, Shallow WGS can be used to build a custom reference panel for a specific population.
Advantages
- More cost-effective than genotyping arrays
- Higher specificity and new rare variant discovery
- Flexible setup of new species or custom populations
SMARTLife Biosciences provides accurate and cost-effective shallow whole genome sequencing and bioinformatics analysis for genomes up to 190 Mb.
We perform DNA barcoding & pooling, library construction for long-read sequencing, sequencing, raw data quality control, and bioinformatics analysis. In addition, we can tailor on request this pipeline to your research interest.
Starting material requirements
- At least 2O µL of purified genomic DNA at 100 ng/µL
- Genome reference sequence (.fasta)
- For genome size up to 190 Mb (ie. Drosophilia, C. Elegans, Arabidopsis Thaliana etc)
Results
SMARTLife Biosciences provides shallow whole genome sequencing service package including DNA extraction, library construction, sequencing, raw data quality control, and bioinformatics analysis. We can tailor this pipeline to your research interest.
If you have additional requirements or questions, please feel free to contact us support@smartlifebiosciences.com.
- Raw data and quality score
- Table (.txt) listing sequencing coverage & quality score at each position of the genome for variant discovery
- Data analysis report
