FULL LENGTH 16S SMARTSEQ
16S/ 18S
ITS
METAGENOMICS
Microbiota composition of your Sample at species resolution level by full length long-read sequencing of 16S rDNA
Ask SMARTLife for the Bacterial composition of your sample at species resolution level
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- Full length 16S rDNA long-read sequencing
- Available from 1 sample to a batch of samples
- Starting from a large variety of samples (water, soil, feces, skin, etc…)
- Differentiation of closely related species
- High accuracy ( 99.6%)
- Comprehensive report (bacterial composition of your sample, relative proportion
SMARTLife services includes :
- DNA extraction (depending on the sample type – ask SMARTLife Support Team here)
- Bacterial ful-length 16S rDNA sequence amplification with V1-V9 full length ( about 1.45 kbp) 16S rRNA gene primers
- Library preparation for Nanopore Long-read sequencing
- Concentration measurements with Qubit dsDNA HS
- Samples barcoding, tagging and equimolar pooling
- Nanopore sequencing using latest chemistry available (R10.4.1) for highest accuracy
- Fast5 raw data acquisition and basecalling using ultra-high quality mode for highest data quality
- Reads demultiplexing and adapters trimming
- Bioinformatics analysis & report generation (read statistics, 16S rDNA read classification, mapping against available database (ie. SILVA), relative abondance calculation, statistical tests and graphical representation)
How to order
It is very easy to order your samples sequencing online.
Full Length Metagenomics SMARTSeq step by step :
- Prepare your purified DNA in line with our concentration & volume requirements
- Label each sample with Green Long read SMARTSeq IDs
- Connect to your SMARTAccount online
- Select QUICK ORDER / Long read
- Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
- Protect your samples in a SMARTBox and write on the back of the box your name and order number
- Contact us for local pick up
Results
- SMARTLifes provide you with your resultats (raw-data inc. quality scores for each sample, report) via your personal & secure OneDrive.
- We store your data for 1 month
