Genome SMARTSEQ
Sequence your bacterial, viral, or yeast genome
Choose the best sequencing service and order now.
We can sequence entire plasmids using 3rd generation technology, based on reading long fragments. We offer different sequencing categories depending on the size of your samples. By choosing our long read sequencing service, you have access to qualitative sequencing and solid bio informatics analysis.
Advantages
- Available from 1 bacterial genome to a batch of genomes
- Valid for De Novo sequencing or Comparative genomic approaches
- Deep sequencing coverage (at least 30X)
- Long read (up to 300 kb)
- High quality of results (>99.6% base accuracy)
- Ultra fast delivery time
- 3d generation sequencing technology
- Amplification free, no PCR bias
- Resolve GC rich and homopolymers regions
- High quality de novo assembly
- Detect all types of variants (from SNPs to large structural variants)
- No need of sequencing primers
- Real time signal acquisition
How to order
It is very easy to order your samples sequencing online.
Genome SMARTSeq step by step :
- Prepare your bacterial genomic DNA in line with our concentration & volume requirements
- Label each sample with Red Long read SMARTSeq IDs
- Connect to your SMARTAccount online
- Select QUICK ORDER / Long read
- Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
- Protect your samples in a SMARTBox and write on the back of the box your name and order number
- Contact us for local pick up support@smartlifebiosciences.com
Concentration & Volume Requirements
Bacterial Genome
Viral Genome
Yeast Genome
Results
SMARTLife Biosciences deliver the WHOLE consensus sequence of bacterial genome and provides you with the following results:
When you do not provide us with a REFERENCE sequence in .fasta format :
– We follow a GENOME DE NOVO sequencing approach.
– We deliver you with the bacterial genome consensus sequence after De Novo assembly performed by proprietary BioIT pipeline ISO13485 compliant.
You receive on personal & secure OneDrive :
- Raw data (text sequences & quality scores in fastq. file)
- Consensus sequence (.fasta file) obtained after De Novo assembly
- Alignement of the reads produced to the consensus sequence (.bam file)
- Structural annotations (assembly.gbk, genes.fasta,proteins.faa)
- Quality metrics & histograms
When you provide us with a reference sequence in .fasta format :
– We follow a COMPARATIVE GENOMICS sequencing approach.
– We deliver you with the bacterial consensus sequence after aligning quality trimmed & filtered long reads to your reference sequence. Alignement is performed with proprietary BioIT pipeline ISO13485 compliant.
You receive on personal & secure OneDrive :
- Raw data (text sequences & quality scores in fastq. file)
- Consensus sequence (.fasta file) obtained after alignement to the reference sequence
- Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
- Structural annotations (assembly.gbk, genes.fasta,proteins.faa)
- Quality metrics & histograms
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