DNA Pool SMARTSeq™ is an advanced long-read sequencing service designed to characterize complex DNA mixture, pooled amplicons, CRISPR-edited amplicons, mixed libraries and heterogeneous DNA samples through direct sequencing of pooled DNA molecules.

Powered by Oxford Nanopore long-read sequencing technology, DNA Pool SMARTSeq™ enables the analysis of DNA fragments ranging from 100 bp to over 300 kb, providing a comprehensive view of both homogeneous and heterogeneous molecular populations. Unlike conventional approaches that rely on clone isolation, DNA Pool SMARTSeq™ directly interrogates every molecule within your sample, preserving the true biological diversity of the population.

Combined with SMARTLife Biosciences’ proprietary BioIT pipelines and local laboratory expertise, DNA Pool SMARTSeq™ delivers rapid and actionable insights into complex DNA mixtures, CRISPR genome editing outcomes, pooled amplicon libraries, structural variants, insertions, deletions and recombination events.

For CRISPR applications, DNA Pool SMARTSeq™ can be integrated with our dedicated CRISPR Analysis Pipeline to provide quantitative classification of wild-type, knock-in, HDR-compatible, NHEJ and complex editing events directly from pooled edited cells, eliminating the need for colony picking or labor-intensive screening workflows.

Key benefits include:

• Direct sequencing of pooled DNA samples without prior cloning
• Quantitative characterization of complex DNA populations
• Detection  and quantification of CRISPR
• End-to-end workflow from library preparation to bioinformatics interpretation
• Fast turnaround time, with results typically delivered within 48–72 hours
• Publication-ready reports and scientific support from experimental design to data interpretation

By combining high-resolution long-read sequencing with advanced bioinformatics, DNA Pool SMARTSeq™ maximizes the probability of identifying biologically meaningful and actionable information from CRISPR experiments, pooled amplicon projects and complex DNA mixtures.

DNA Pool Long Read SMARTSeq is suitable for simple to complex mixtures. From 5,000 reads / aliquot to multiple of 5,000 reads from each vial depending on the complexity of your intial DNA mixture or the frequency of CRISPR edit you're interested in.

Long Read Nanopore sequencing is performed from native DNA molecules from your startng material, without any amplification step. This streamlined process provides a much more efficient and scalable approach than Sanger sequencing signal deconvolution, especially when dealing with complex DNA mixtures..

Our local laboratories perform Long Read SMARTSeq sequencing directly on native DNA from your vial.

The sequencing run is calibrated to generate up to 5,000 Long Reads / sample or multiple of up to 5,000 reads ( depending on starting material available in the vial).