SMARTLife Biosciences established and validated the DNA Pool Long Read SMARTSeq sequencing service for various applications. Combined with expert bioinformatics, it enables accurate and precise characterization of CRISPR-induced edits directly across your target locus. Combined with clustering & expert bioinformatics, it enables the identification of the different components of DNA mixtures and the characterization of their similarities and differences.

DNA Pool Long Read SMARTSeq is suitable for simple to complex mixtures. From 5,000 reads / aliquot to multiple of 5,000 reads from each vial depending on the complexity of your intial DNA mixture or the frequency of CRISPR edit you're interested in.

Long Read Nanopore sequencing is performed from native DNA molecules from your startng material, without any amplification step ( mix of amplicons or mix of plasmids, phagemids etc). This streamlined process provides a much more efficient and scalable approach than Sanger sequencing signal deconvolution, especially when dealing with complex DNA mixtures..

Our local laboratories perform Long Read SMARTSeq sequencing directly on native DNA from your vial.

Results are available in 48 to 72h (depending on samples batch size).

The sequencing run is calibrated to generate 5,000 Long Reads / sample or multiple of 5,000 reads depending on the DNA mixture complexity ( depending on starting material available in the vial).

Thanks to the long read length (from 100 bp to 300 kb), this service allows for the direct sequencing of pool of DNAs molecules like amplicons mixture ensuring accurate identification of CRISPR edits for instance.