SMALL Amplicon SMARTSeq
SMALL Amplicon SMARTSeq
15,00€
Select the number of each type of sample you want to sequence.
IMPORTANT : To sequence and analyse your samples, our laboratory needs all the details asked in the Sample Sheet.
1. Download and fill in the SampleSheet.
2. Drag & drop SampleSheet + Reference below in case you have a Reference in fasta format (.fasta or .fa) available.
Providing us with a Reference improves the quality of the Bioinformatic analysis.
3. Place your order.
DO NOT FORGET to fill in the Sample Sheet and upload it with your order.
SMALL linear DNA / Amplicon PCR (300 bp-25 kb) 3d Generation sequencing producing long reads (up to 300 kb).
High quality of results are delivered via your personal & secure One Drive with ultra short delivery time.
- Prepare a volume >= 10 uL of your purified plasmid double strand circular DNA at 30 ng/ uL in Eppendorf tube (1.5 mL).
- Label your tube with a Blue long read SMARTSeq lDs.
- Connect to your SMARTAccount online.
- Select QUICK ORDER / Long read and define the number, type, size of the samples, upload your SampleSheet and your reference sequence in .fasta
- Place your samples in a SMARTBox and write on the back your name and order number.
- Contact us for local pick up (support@smartlifebiosciences.com)
You receive via your personal & secure One Drive :
- Raw data ( text sequences & quality scores (.fastq file)
- Alignment (.bam) against your reference sequence in case you provided us with a reference sequence in .fasta format
- Table of all variants detected by comparison to your reference sequence (.tsv and xlsx files)
To improve the quality of the BioIT analysis, we recommend to provide us with a reference sequence. In case you didn't provide us with a reference sequence, we perform an auto-assembly of produced reads with our proprietary pipeline and deliver you a consensus sequence (.fasta) and corresponding table with consensus base quality and coverage at each position of the consensus (.tsv and xlsx).