AMPLICON SMARTSEQ
Sequence your PCR product
Choose the best sequencing service for your linear DNA and order now.
Using 3rd generation technology, we can sequence from small to very long DNA fragments. We offer different sequencing categories depending on the size of your samples. By choosing our long read sequencing service, you have access to qualitative sequencing and solid bio informatics analysis.
We can sequence SMALL amplicons (< 450 bp to 25 kb), BIG amplicons (25 to 75 kb) and HUGE amplicons (75 to 125 kb) in less than 24 hours.
Advantages
- Available from 1 sample to 96 samples
- High quality of results (99.6% accuracy)
- Deep sequencing coverage (> 30X)
- Long read for complete sequencing of your linear DNA
- Real time data acquisition
- Ultra fast delivery time
- Amplification free, no PCR bias
- No need of sequencing primer
- No sample transportation, local laboratories
- Detect all variants : from SNPs to large structural variation
- Identify contaminants in your preparation
- Proprietary BioIT pipelines
- Custom BioIT services available on request
How to order
It is very easy to order your samples sequencing online.
Amplicons SMARTSeq step by step :
- Prepare your purified double strand circular plasmid DNA in line with our concentration & volume requirements
- Label each sample with Blue Long read SMARTSeq IDs
- Connect to your SMARTAccount online
- Select QUICK ORDER / Long read
- Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
- Protect your samples in a SMARTBox and write on the back of the box your name and order number
- Contact us for local pick up support@smartlifebiosciences.com
Don’t limit yourself.
Obtain the COMPLETE sequence of amplicon PCR.
Detect all variants with 3rd generation long read sequencing.
Results
SMARTLife Biosciences produces long reads (30X coverage) and performs an alignment to your reference sequence with a proprietary bio-IT pipeline. You receive in your personal & secure One Drive the following results:
-
- Raw data (text sequences & quality scores (fastq. file)
- Consensus sequence (.fasta file)
- Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
- Table of detected variants compared to the reference sequence (.vcf file)
- Quality metrics & histograms
Discover as well