LOW PASS
WHOLE GENOME SEQUENCING
Genotyping by Long-read sequencing
Genotype-by-long-read-sequencing is a very attractive and cost-effective approach for genome characterization or genotyping. It requires a low sequencing depth (1X to 5X) of genome size up to 200 Mb to be cost effective. Low-pass whole genome sequencing (also referred to as shallow whole genome sequencing) is used to detect genetic variation. This method is an affordable alternative for discovering new, rare variants with higher statistical power and increased data. Low Pass Whole Genome Long read sequencing helps to address questions related to statistical and population genetics, from complex-trait association studies to genome-wide polygenic risk score calculation.
Shallow WGS returns more data, greater statistical power, and new rare variant discovery capabilities than traditional genotyping arrays. Shallow WGS can be used in genome-wide association study (GWAS), evolutionary analysis, pharmacogenomics, molecular breeding, etc. In addition, Shallow WGS can be used to build a custom reference panel for a specific population.
Advantages
- More cost-effective than genotyping arrays
- More data, greater statistical power, and new rare variant discovery capabilities than classical genotyping arrays
- Flexible setup of new species or custom populations
SMARTLife Biosciences provides accurate and cost-effective shallow whole genome sequencing and bioinformatics analysis for large genomes.
We perform DNA barcoding & pooling, library construction for long-read sequencing, sequencing, raw data quality control, and bioinformatics analysis. In addition, we can tailor on request this pipeline to your research interest.
Starting material requirements
- At least 2O µL of purified genomic DNA at 100 ng/µL
- Genome reference sequence (.fasta)
- For genome size up to 190 Mb (ie. Drosophilia, C. Elegans, Arabidopsis Thaliana etc)
Results
CD Genomics provides full shallow whole genome sequencing service package including DNA extraction, library construction, sequencing, raw data quality control, and bioinformatics analysis. We can tailor this pipeline to your research interest.
If you have additional requirements or questions, please feel free to contact us support@smartlifebiosciences.com.
- Raw data and quality score
- Table (.txt) listing sequencing coverage & quality score at each position of the genome for variant discovery
- Data analysis report
