Bacterial Genome SMARTSEQ

BACTERIAL GENOME SMARTSEQ


  • Available from 1 bacterial genome to a batch of genomes
  • Valid for De Novo sequencing or Comparative genomic approaches
  • Deep sequencing coverage (at least 30X)
  • Long read (up to 300 kb)
  • High quality of results (>99.6% base accuracy)
  • Ultra fast delivery time
  • Check our SMART guide to prepare your samples
  • Order Long read SMARTSeq IDs to label your samples
3d generation long read sequencing of bacterial genome
3d generation long read sequencing of bacterial genome
  • 3d generation sequencing technology
  • Amplification free, no PCR bias
  • Resolve GC rich and homopolymers regions
  • High quality de novo assembly
  • Detect all types of variants (from SNPs to large structural variants)
  • No need of sequencing primers
  • Real time signal acquisition

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Bacterial Genome SMARTSeq step by step :

  1. Prepare your bacterial genomic DNA in line with our concentration & volume requirements
  2. Label your sample with Red long read SMARTSeq lD.
  3. Connect to your SMARTAccount online.
  4. Select QUICK ORDER / Long read.
  5. Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
  6. Protect your samples in a SMARTBox and write on the back of the box your name and order number.
  7. Contact  us for local pick up support@smartlifebiosciences.com

SMARTLife Biosciences deliver the WHOLE consensus sequence of bacterial genome and provides you with the following results:

When you do not provide us with a REFERENCE sequence in .fasta format :

– We follow a GENOME DE NOVO sequencing approach.

– We deliver you with the bacterial genome consensus sequence after De Novo assembly performed by proprietary BioIT pipeline ISO13485 compliant.

You receive on personal & secure OneDrive :

  • Raw data (text sequences & quality scores in fastq. file)
  • Consensus sequence (.fasta file) obtained after De Novo assembly
  • Alignement of the reads produced to the consensus sequence (.bam file)
  • Structural annotations (assembly.gbk, genes.fasta,proteins.faa)
  • Quality metrics & histograms

When you provide us with a reference sequence in .fasta format :

– We follow a COMPARATIVE GENOMICS sequencing approach.

– We deliver you with the bacterial consensus sequence after aligning quality trimmed & filtered long reads to your reference sequence. Alignement is performed with proprietary BioIT pipeline ISO13485 compliant.

You receive on personal & secure OneDrive :

  • Raw data (text sequences & quality scores in fastq. file)
  • Consensus sequence (.fasta file) obtained after alignement to the reference sequence
  • Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
  • Structural annotations (assembly.gbk, genes.fasta,proteins.faa)
  • Quality metrics & histograms