PLASMID SMARTSEQ

PLASMID SMARTSEQ


Available from 1 sample to 96 samples

High quality of results (99.6% accuracy)

Deep sequencing coverage (> 30X)

Long read (up to 300 kb)

SMART guide to prepare your samples

Long read SMARTSeq IDs to label your samples

  • 3d generation sequencing technology
  • Amplification free, no PCR bias
  • Real time data acquisition
  • No need of sequencing primer
  • No sample transportation, local laboratories
  • Detect all variants : from SNPs to large structural variation
  • Identify contaminants in your preparation

Plasmid SMARTSeq step by step :

  1. Prepare your purified double strand circular plasmid DNA in line with our concentration & volume requirements
  2. Label each sample with Blue Long read SMARTSeq IDs.
  3. Connect to your SMARTAccount online.
  4. Select QUICK ORDER / Long read.
  5. Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
  6. Protect your samples in a SMARTBox and write on the back of the box your name and order number.
  7. Contact  us for local pick up support@smartlifebiosciences.com

SMARTLife Biosciences reconstituted the COMPLETE sequence of your construct and provides you with the following results:

  1. Raw data (text sequences & quality scores (fastq. file)
  2. Consensus sequence (.fasta file)
  3. Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
  4. Plasmid circular map & annotations (.pLannote file)
  5. Quality metrics & histograms
Histogram of quality scores

Preparation with several pics: presence of contaminants (concatemers, deletions, recombinations, etc.).

Preparation with a single circular plasmid.

Annotated vector map inc. features table

Save time & money and secure the success of your experiment at an early stage.
Verify upfront the complete sequence of your construct by long read sequencing.