Available from 1 sample to 96 samples

High quality of results (99.6% accuracy)

Deep sequencing coverage (> 30X)

Long read (up to 300 kb)

SMART guide to prepare your samples

Long read SMARTSeq IDs to label your samples

  • 3d generation sequencing technology
  • Amplification free, no PCR bias
  • Real time data acquisition
  • No need of sequencing primer
  • No sample transportation, local laboratories
  • Detect all variants : from SNPs to large structural variation
  • Identify contaminants in your preparation

Plasmid SMARTSeq step by step :

  1. Prepare your purified double strand circular plasmid DNA in line with our concentration & volume requirements
  2. Label each sample with Blue Long read SMARTSeq IDs.
  3. Connect to your SMARTAccount online.
  4. Select QUICK ORDER / Long read.
  5. Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
  6. Protect your samples in a SMARTBox and write on the back of the box your name and order number.
  7. Contact  us for local pick up

SMARTLife Biosciences reconstituted the COMPLETE sequence of your construct and provides you with the following results:

  1. Raw data (text sequences & quality scores (fastq. file)
  2. Consensus sequence (.fasta file)
  3. Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
  4. Plasmid circular map & annotations (.pLannote file)
  5. Quality metrics & histograms
Histogram of quality scores

Preparation with several pics: presence of contaminants (concatemers, deletions, recombinations, etc.).

Preparation with a single circular plasmid.

Annotated vector map inc. features table

Save time & money and secure the success of your experiment at an early stage.
Verify upfront the complete sequence of your construct by long read sequencing.