SMALL Pool DEEP Long Read SMARTSeq is designed for Mixtures of DNAs like Binders library , Antibody library, pool of different clones, pool of Amplicons, etc.

- Valid for long read Nanopore sequencing of a mixture of circular (ie. pool of plasmides or phagemides) or linear (pool of Amplicons) DNAs pooled in one vial.

- Production of 5,000 reads from your vial

- Suitable for SMALL MIX corresponding to low to moderate complexity of  DNAs mixture.

- Available for DNAs size ranging between 3 - 10 kb in case of circular DNAs and between 100 bp to 5 kb in case of linear DNAs.

- Available for 1 vial or a batch of vials.

Key Benefits :

• Full-Length Sequences: Obtain the complete sequences of your DNAs fragments, avoiding gaps and ambiguities that can arise with short-read technologies.
• Improved Accuracy: Accurately sequence repetitive or homopolymeric regions that are often difficult to resolve with shorter reads, ensuring more reliable results.
• High-Resolution Detection: Detect rare targets present in your mix that might otherwise be missed with other sequencing methods.
• Streamline process: no need of plating and picking, sequencing is performed directly on the native DNAs mix present in your vial
• Versatility: Ideal for a wide range of applications, including Binder library QC, Antibody library Deep Mining, clonal evaluation, synthetic constructs check, etc.

When to Use This Service:

• Binders library QC: for the validation and quality assessment of a library of DNA fragments that encode molecular binders ( antibodies, aptamers, peptide binders, etc)
• Antibody library Deep Mining: for the comprehensive analysis and discovery of rare or enriched antibody sequences within large, diverse libraries. Instead of just selecting binders through traditional rounds of panning and screening, deep mining aims to capture the full diversity and dynamics of the antibody population, especially low frequency target, and full length antibody sequences including both VH and VL chains and CDR regions.
• Clonal library evaluation : to determine different clones proportion in a library from synthetic design and to confirm sequence integrity
• Amplicons PCR pool : for rare variant detection, random mutagenesis libraries, CRISPR guide-specific amplicons check

This service is ideal for project requiring Deep sequencing, full-length sequence data, demultiplexing and quantification, ensuring you get the most comprehensive and reliable results possible. Whether you're conducting Binders library QC, Antibody libraries Deep Mining, Cloncal library evaluation, rare variant detection or random mutagenesis, our DNA Mix Deep Long-Read Sequencing service will provide the depth and accuracy your project requiers.

Ask SMARTLife for SMALL Pool DEEP Long Read SMARTSeq :

• Local Team Ready to Listen to your projects
• Fast Service with 48 to 72-Hour Results Delivery (depending on samples batch size)
• Comprehensive and Understandable Results
  • 5,000 Long reads (5K) for small mixture complexity
  • Raw data ( text sequences & quality scores (.fastq file)
  • Alignment (.bam) against your reference sequence in case you provided us with one reference sequence in .fasta format and table of all variants detected by comparison to your reference sequence (.tsv and xlsx files)
  • Alignment of multiple reference sequences available as an add-on service

Simplify your admin ! Place 1 order for a batch of prepaid barcodes and sequence when your vial is ready.