Long-Read Sequencing Service for Amplicon/PCR Analysis

Our Long-Read Sequencing service for Amplicon/PCR analysis provides a powerful tool for generating accurate, full-length sequences of amplified regions, offering a comprehensive view of your target genes or genomes. Unlike traditional short-read methods, long-read sequencing can capture entire amplicon sequences in a single read, minimizing errors associated with repetitive regions and enabling more reliable interpretation of complex genomic data. This service is ideal for applications where precision and full-length coverage are critical, such as studying gene variants, microbial diversity, or specific genomic regions.

Key Benefits:

• Full-Length Amplicon Sequences: Obtain complete and uninterrupted sequences of your PCR products, avoiding the gaps and ambiguities that can arise with short-read technologies.
• Improved Accuracy: Accurately sequence repetitive or homopolymeric regions that are often difficult to resolve with shorter reads, ensuring more reliable results.
• High-Resolution Detection: Detect rare mutations, insertions, deletions, and structural variations within amplicons that might otherwise be missed with other sequencing methods.
• Simplified Data Analysis: The longer reads allow for a more straightforward assembly, reducing the need for complex error correction or post-sequencing refinement.
• Versatility: Ideal for a wide range of applications, including targeted gene sequencing, metagenomic analysis, and microbiome research.

When to Use This Service:

• Gene Variant Analysis: For studying specific genetic regions, such as exons, promoters, or other functional elements, where a full-length sequence is essential for accurate variant detection.
• Structural Variant Detection: If your research requires the identification of structural variations or larger genomic rearrangements, long-read sequencing ensures these can be captured with greater precision.
• Rare Mutations or Variants: For cases where detecting low-frequency or rare variants within a target gene or region is critical to your research.

This service is ideal for any project requiring high-resolution, full-length sequence data of PCR-amplified regions, ensuring you get the most comprehensive and reliable results possible. Whether you're conducting gene expression analysis, studying genetic diversity, or exploring microbiome dynamics, our Long-Read Sequencing service will provide the depth and accuracy your project demands.

Ask SMARTLife for the whole plasmid sequencing of your sample :

• Local Team Ready to Listen to your projects
• Fast Service with 24-Hour Results Delivery
• Comprehensive and Understandable Bioinformatics Results
  • Raw data ( text sequences & quality scores (.fastq file)
  • Consensus sequence (.fasta) from proprietary auto-assembly pipeline and table with consensus base quality and coverage at each position of the consensus (.tsv and xlsx)
  • Alignment (.bam) against your reference sequence in case you provided us with a reference sequence in .fasta format and table of all variants detected by comparison to your reference sequence (.tsv and xlsx files)