POOL DEEP-SMARTSEQ
Sequence DNAs POOL
Choose the best sequencing service for your DNAs POOL and order now.
We can sequence your mixture of circular or linear DNAs using 3rd generation Nanopore technology. Our local laboratories produce different sequencing depths ( 5,000 reads/ replicate) depending on the number of replicates we prepare from your library. We can sequence DNA pools of varying complexity by adjusting the sequencing depth produced from your vial.Readlength can be up to 300 kb (depending on starting material quality).
This service is available for circular DNAs (plasmids or phagemids < 10 kb), or linear DNAs pool ( from 100 bp to 5 kb). We can explore library of various complexity by adjusting the sequencing depth produced from your vial. To do so, we produce a number of replicates (duplicates, triplicates, etc.) and we produce from each replicate approximately 5,000 reads, allowing us to deliver deeper coverage as needed for your Research project. By selecting our POOL DEEP SMARTSeq service, you have access to meaningful and high quality of results. Reads demultiplexing and quantification can be done on request with Custom Bioinformatics add-on service at extra cost.
Advantages
- Available from 1 sample to several samples
- Sequencing depth scalable (5,000 reads, 10 000, 15000, etc. ) depending on your project objectives and the complexity of your DNAs pool.
- High quality of results (99.6% accuracy)
- Sequencing coverage adjusted to your Research objectives
- Long reads (up to 300 kb)
- Sequencing of native DNAs
- Amplification free, no PCR bias
- No need of sequencing primer
- No sample transportation, local laboratories
- Can detect rare DNA fragments in your mix
- Demultiplexing and quantification of each DNAs population provided as Custom Bioinformatics available upon request
How to order
It is very easy to order your samples sequencing online.
DNAs POOL DEEP SMARTSeq step by step :
- Prepare your aliquot of purified double strand circular or linear DNA pool in line with our concentration & volume requirements
- Label each sample with Purple Long read SMARTSeq IDs
- Connect to your SMARTAccount online
- Select QUICK ORDER / Long read
- Define the number of reads (1 aliquot for 5000 reads) you want to order depending on the complexity of your library, and upload your SampleSheet and your reference sequence in .fasta
- Protect your aliquot in a SMARTBox and write on the back of the box your name and order number
- Contact us for local pick up at support@smartlifebiosciences.com or scan the QR code available on your Institut FridgeBox when you drop your samples off.
Get more for your money.
Secure Deep Mining of your library.
Get deep, full length sequencing from your DNAs mix with long read sequencing.
Results
SMARTLife Biosciences local laboratory produces at least 5000, and provides you with the following results:
- Raw data (text sequences & quality scores (fastq. file)
- Alignment to one Reference sequence if you provide us with a Reference (.bam file). In case you have several References, multiple alignment can be performed on request for a small additional fee.
- Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
- Reads Demultiplexing and Quantification can be performed on request with Custom Bioinformatics for a small additional fee.
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