CRISPR Amplicon SMARTSeq DEEP (100X)

CRISPR Amplicon SMARTSeq DEEP (100X)

  • Available from 1 sample to 96 samples
  • Sequencing of up to 25 kb CRISPR/Cas linear amplicon
  • DEEP (100X) sequencing coverage for precise visualization of targeted loci and clivage sites
  • Adjusted protocol with high quality base-calling dedicated to variants detection
  • Long read (up to 300 kb) to sequence entirely your linear DNA/ Amplicon
  • Ultra short delivery time
  • SMART guide to prepare your samples
  • Long read SMARTSeq IDs to label your samples
  • Full length sequence of targeted loci
  • Save cloning time & sequence directly your CRISPR amplicons (up to 25 kb)
  • Effective transgene mapping with complex integration loci
  • Detect all classes of variants : from SNPs to complex non-coding structural variants
  • 3d generation sequencing technology
  • Amplification free, no PCR bias
  • No need of sequencing primer
  • Real time signal acquisition

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Linear / Amplicon SMARTSEQ step by step :

  1. Prepare your CRISPR/Cas purified linear DNA / amplicon up to 25kb according to our recommendations 
  2. DEEP SMARTSeq (100X sequencing coverage) requiers more DNA quantity ( >= 10 uL at 80 ng/uL) than REGULAR SMARTSeq sequencing (30X sequencing coverage).
  3. Label your tube with a Green long read SMARTSeq lDs.
  4. Connect to your SMARTAccount online.
  5. Select QUICK ORDER / Long read
  6. Define the number, type, size of your sample, and upload your SampleSheet and your reference sequence in .fasta
  7. Protect your samples in a SMARTBox and write on the back of the box your name and order number.
  8. Contact  us for local pick up

For CRISPR/Cas project, SMARTLife Biosciences produces REGULAR (30X) or DEEP (100X) sequencing coverage and long reads (up to 300 kb) . Long reads are aligned to the reference genome with a proprietary bio-IT pipeline. You receive in your personal & secure One Drive the following results:

  1. Raw data (text sequences & quality scores (fastq. file)
  2. Consensus sequence (.fasta file)
  3. Table of consensus base quality & coverage at each position (.tsv and .xlsx files)
  4. Table of detected variants compared to the reference sequence (.vcf file)
  5. Quality metrics & histograms

Visualisation of bases quality score with SnapGene Viewer

Vizualisation of long reads alignment to reference genome with SnapGene Viewer

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